Screening and Preliminary Identification of Soil Potassium and Phosphorus-hydrolyzing Bacteria WP1-3

In order to screen the strains with Potassium and phosphorus-releasing bacteria from soil, potassium-dissolving medium was used for preliminary screening, transparent circle method re-screening. The content of soluble potassium determined by sodium tetraphenylborate method, phosphorus anticolorimetric molybdenum antimony. were identified morphological, biochemical molecular biology. results showed that ten potassium-solubilizing function obtained screening solubilizing medium. re-screening WP1-3 strain had strongest ability, D/d value 1.33. decomposition is 22.918 mg/L, decompose inorganic organic ability 4.443 mg/L 22.408 respectively. Identifying morphological identification gram-negative bacteria, not produce spore, nitrogen increased after detection culture 3d 7d 10.825 38.521 mg/L. 16SrDNA size 1351bp, ribosomal database comparison 99.8% similar Rhizobium. Therefore, belongs rhizobia genus.